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RAS BiologyБиология внутренних вод Inland Water Biology

  • ISSN (Print) 0320-9652
  • ISSN (Online) 3034-5227

Some Characteristics of Three Strains of the Black Sea Algal Viruses and Their Impact on Planktonic Microalgae

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PII
S30345227S0320965225050096-1
DOI
10.7868/S3034522725050096
Publication type
Article
Status
Published
Authors
R.R. Sagadatova
  • Affiliation: Kovalevsky Institute of Biology of the Southern Seas of Russian Academy of Sciences
Volume/ Edition
Volume 18 / Issue number 5
Pages
869-882
Abstract
Three strains of algal viruses (TvV-SM2, IgV-SS1, EhV-SS2) were isolated from the coastal waters of the Black Sea in 2022–2023. The first strain caused lysis of cells of the green algae and , the second affected cells of the pyrimesiophyte , and the third lysed the coccolithophore . No other hosts of the pathogens were detected during the study of possible contact of the isolated viruses with indicator cultures of 32 species of marine microalgae. Viral particles of all three strains had the shape of a regular convex icosahedron, and their diameter ranged from 48 to 174 nm. They were found to have a second membrane – a supercapsid. The titer of TvV-SM2 was 1.3 × 10 virions/ml, IgV-SS1 and EhV-SS2 – 3.1 × 10 and 2.5 × 10 virions/ml, respectively. The effect of different concentrations of copper ions on the activity of algal viruses was studied for the first time using TvV-SM2 as an example. Under the influence of the toxicant at a concentration of 100 µg/l, complete suppression of the pathogen was revealed. When TvV-SM2 algal virus affected , the latent period of infection was 24 h, and the rate of algal cell lysis was equal to 2.59 day on average. Upon contact of EhV-SS2 algal virus with culture, the latent period increased by 4.2 times, and the lysis rate decreased by almost an order of magnitude. During the latent period of infection, a 1.7–2-fold decrease in the efficiency of photosystem II was observed in both infected cultures. By the end of the experiments, an average of 10% of algal cells were not lysed.
Keywords
Черное море альговирусы микроводоросли Array Array лизис культуры
Date of publication
08.12.2025
Year of publication
2025
Number of purchasers
0
Views
35

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